Institute of Endemic Diseases - SUDAN

 

 
 

 

CURRICULUM VITAE

Name:  Hiba Salah-Eldin Mohamed               

Sex: Female

Place of Birth:  Tuti Island/ Khartoum/ Sudan

Nationality:  Sudanese

Language: Arabic/English

Address:  Institute of Endemic Diseases. University of Khartoum. P. O. Box 102. 

E. mail hibasalah@iend.org:  Fax:  002491-83779712.

Tel. Office: 002491-83793268, Mobile 0912605221

Qualification

 Ph.D in Molecular Biology –June -2002.  Institute of Endemic Diseases. University of Khartoum

Current Post

Assistant Professor, Department of Molecular Biology. Institute of Endemic Diseases. University of Khartoum

Education

  • 1975-1987:  Tuti Primary, Intermediate and Higher Secondary Schools
  • 1987- 1993:  BSc.(Hons), Second Class Division One, Zoology, faculty of Science, University of Khartoum
  • 1995-1997:   MSc. Zoology, University of Khartoum entitled: A Cross-sectional Study of Cutaneous Leishmaniasis in Tuti Island Supervised by Prof Ahmed M. El Hassan and Dr Sadia A Youins.
  • 1998-2002:   Ph.D, Molecular Biology.  Institute of Endemic Diseases University of Khartoum entitled: The role of Host Genetics in Susceptibility to Kala-azar in The Sudan. Supervised by:  Prof. J.Blackwell, University of Cambridge and Prof. Muntaser E. Ibrahim.  Institute of Endemic Diseases, University of Khartoum.

Last Post Held

  • 1993-1996:   Teaching assistant on part time bases (Faculty of Science, Faculty of   Education, University of Khartoum- University of Juba- University of Bahr El Gazal)
  • 1994-1997:   Teaching assistant University of El Neelain, Sudan
  • 1998-2000:   Lecturer, University of El Neelain, Sudan
  • 2000- 2002:  Lecturer.  Institute of Endemic Diseases, University of Khartoum
  • 2002-2003: Research assistant during my PhD in the Wellcome Trust Collaborative Grant (Role of Host genetics in Susceptibility to Kala-azar In Sudan) between university of Khartoum (Dr. Muntaser E. Ibrahim ) and University of Cambridge ( Prof. Blackwell).
  • March-October 2002:  Post doctoral fellowship. Cambridge Institute for Medical Research. University of Cambridge.
  • June 2002- till now Assistant Professor-Department of Molecular Biology –Institute of endemic Diseases, University of Khartoum.

Ongoing and Conducted Researches

  • Frequency of sugar feeding of Phlebotomus Papatasi in Surogia area. (1993). Graduation dissertation. Supervisor Dr. Dia Eldin Elnaiem.  Faculty of Science University of Khartoum   
  • Epidemiological study of cutaneous leishmaniasis in Tuti Island-Khartoum looking for the prevalence of the disease in human and sandflies (MSc-Research)-1996-1998)
  • Genetic susceptibility to visceral leishmaniasis –Candidate gene approach (1998-2002). PhD research
  • Diagnosis of leishmaniasis using PCR  (1996-1998)
  • Functional and Genetic analysis of NRAMP1 gene using Real time PCR, reporter assay and sequencing (2003-2007).
  • Looking for possible reservoir host for visceral leishmaniasis by investigating natural infection and experimental infection in dogs (2006-ongoing)
  • Immunogenetics of cutaneous leishmaniasis in newly inhabited area in Khartoum (2007-ongoing)
  • Screening of known cytokine, nitric oxide and complement receptor one gene polymorphisms in Sudanese from malaria endemic areas
  • Possible genes control susceptibility to Mycetoma, using SNP chips (On going research.

Main Research Interest

  • Host –Parasite interaction
  • Genetics susceptibility to infectious diseases in particular to visceral leishmaniasis (VL), post-kala-azar dermal leishmaniasis (PKDL) and Malaria
  • Looking for possible functional role of different gene polymorphisms and its role in differential gene expression using Reporter assay, Real time PCR.

Courses and Training

  • Molecular Biology techniques in Research and Diagnosis of Infectious Diseases (1996). Institute of Endemic Diseases, University of Khartoum
  • Biostatistics, using Statistical Package for Social Studies (SPSS) (1997). Tropical Medicine Institute. Sudan 
  • Introduction to Immunology and Molecular Biology (1997).  Institute of Endemic Diseases, University of Khartoum
  • Genotyping and Linkage Analysis Course (1999).  Human Genome Resource Centre. Hinxton. Cambridge. UK
  • Introduction to Bioinformatics (2000). Cambridge University. UK.
  • Training at Cambridge Institute for Medical Research (Part of my Ph.D). (1999-2002). University of Cambridge. Funded by WHO/TDR and Wellcome Trust.
  • WHO/TDR advance course in Immunology and biotechnology for infectious diseases, (2003.). Lausanne- Switzerland, Annecy- France.
  • Training from 2004-2007, three months every year,  at Cambridge Institute for Medical Research- University of Cambridge. UK 
  • Training course for one month at Institute for Staff Skills Development -University of Khartoum (2006)
  • Functional Genomic of Malaria Parasites (Biotec, Thiland ). March -2007.  Funded by WHO/TDR and BioMalPar

·        Best Practices in Ethical Review. 24-26 July 2007. Organized by HAT Platform, Good Clinical Practice Alliance – Europe and Tropical Medicine Institute, NCR, Khartoum-Sudan -Khartoum -Sudan

Grants Awarded

  • WHO/ TDR training fellowship for 8 months (1999) in Cambridge University
  • Wellcome Trust Research Development Award (2004-2007). Genetics and Functional analysis of SLC11A1 (formerly NRAMP1) polymorphisms in susceptibility to visceral leishmaniasis in the Sudan.  In collaboration with Prof. Blackwell University of Cambridge. UK, (₤90,000) The Award was included (fund for: equipment to the Institute, consumables, visits to Cambridge university 3 months every year for research).
  • University of Khartoum Research fund for one years (2007) (Genetics and immunological factors determining the severity of P. falciparum infection. (5000 SDG)
  • WHO capacity strengthening program grant for faculty of Science (2005)              “investigation of possible reservoir host in Eastern Sudan “Co-investigator. 
  • Royal Society Pfizer Award (2007). Recognition of my work on the genetic basis of susceptibility to visceral leishmaniasis in the Sudan. The prize support young African scientist in biomedical field. ₤60,000 fund for the research entitled “Functional Genomic of Visceral leishmaniasis and PKDL in the Sudan”.

Teaching Experiences

  • General Zoology- Practical–first Year (1995-2000) - AL-Neelain University.
  • General Zoology- Practical–first Year (1994-2000)-Faculty of Science- U of K, Faculty of   Education- U of K, University of Juba- University of Bahr El Gazal (on part-time bases)
  • Genetics-Practical – 2nd year - AL-Neelain University.
  • Animal physiology -Practical - 4th Year –AL-Neelain University.

·        Vertebrate course-Theory-Practical– 2nd year- (1999)- AL-Neelain University.

·        Host-parasite interaction- Theory -Third year (1999)-AL-Neelain University.

·        Molecular Biology course - Qualifying year - Institute of Endemic Diseases-University of Khartoum –2002 –to date

·        Molecular biology course -4th year - Al-Neelain University- 2004

·        Statistical Genetics Theory and Practical Institute of Endemic Diseases-2005- to date

·        Cell Molecular Biology course  – Master of Molecular Medicine –Institute of Endemic Diseases- (2007)

·        Genetic susceptibility to infectious diseases- Master degree of molecular medicine –Institute of Endemic Diseases- (2007)

·        Statistical Genetics- Master of molecular medicine –Institute of Endemic Diseases (2007).

·        Bioinformatics and statistical genetics. University of Elneelain February –2007

·        Introduction to Bioinformatics course –2006-‏2007‏‏‏

·        Molecular biology -First year- National college for medical and technical studies-2007

Administrative Responsibilities

  • Postgraduate Students Coordinator (2006-present)

Organization of Workshop and Courses

  • Khartoum Winter School of Bioinformatics and Statistical Genetics an annual course -2005-present. Collaboration with University of Cambridge -UK

Capacity Building

The Wellcome Trust Grant:

  • RNA laboratory with: PCR machine, Gel electrophoresis system, Microcentifuge and pipettes and consumable for RNA extraction and amplification.
  • Cell culture facilities: –20 oC freezer, laminar flow hood, CO2 incubator, distiller, reagents, consumable for cell and parasite culture and training

The Royal Society Award:

·        Functional Genomic lab with:  Real time PCR machine for gene expression and pathogen detection.  Nanodrop machine, for RNA and DNA quantification.  Consumable for functional work and training for students

Contribution in Scientific Meetings

1.      Mohamed HS; M.E.Ibrahim; E.A.G.Khalil;S. A. Younis; E.Salim; E.E.‎Zijlstra and A.M.El Hassan. Molecular Epidemiological Study of Cutaneous Leishmaniasis in Tuti Island.  Institute of Endemic Diseases Meeting 1998- Khartoum -Sudan. Oral Presentation by Mohamed HS. 

    1. Mohamed HS,  Ibrahim ME, Miller EN, Khalil EAG, El Hassan AM, Blackwell JM. (2001). Role of Candidate Genes in Susceptibility to Kala-azar in the Sudan.. Keystone Symposium USA. Poster by Mohamed HS 
    2. Mohamed HS,  Ibrahim ME, Miller EN, Khalil EAG, El Hassan AM, Blackwell JM.  Role of NRAMP1 in Susceptibility to Kala-azar. (2002). The 6th International Meeting on Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, Pasteur Institute, Paris, France. Oral presentation by Mohamed HS
    3. Mohamed HS,  Ibrahim ME, Miller EN, Khalil EAG, El Hassan AM, Blackwell JM Role of Host Genetics in Susceptibility to Kala-azar. (2002). Genetics of the population of the Sudan and East Africa Meeting. Institute of Endemic Diseases. University of Khartoum. Sudan. Oral presentation by Mohamed HS
    4. Salih MA, Ibrahim ME, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2006). IFNG and IFNGR1 Genes Polymorphisms in Susceptibility to Post kala-azar Dermal Leishmaniasis in Sudan. African Society of Human Genetics Meeting –Addis Ababa, Ethiopia- Poster presentation by Salih MA
    5. Saaid BM, ElHassan IM., Ibrahim ME and Mohamed HS. (2005). Genotyping of nitric oxide synthase 2 polymorphisms in different ethnic groups in Eastern Sudan.  4th MIM Pan-African Malaria conference- Cameroon ..  Poster presentation by Saaid BS
    6. Saaid B M, ElHassan IM., Ibrahim ME and Mohamed HS. (2006).  Prevalence of nitric oxide gene polymorphisms in two populations.  XI international Congress of Parasitology ,  Glasgow. United Kingdom. Poster presentation by Saaid MS.
    7. Elshaikh M, Ibrahim ME, Elhassan I, Saaid B and Mohamed HS. (2007) Prevelance of Il-4 and Il-4 receptor genes polymorphisms in Malaria endemic area in Eastern Sudan. Gordon Malaria conference .Oxford. United Kingdom.. Poster presentation by Elshaikh M.        
    8. Farouk S.,Ibrahim ME,Salih MA, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2007). IL10 Gene Polymorphisms and development of Post-Kala-azar dermal leishmaniasis. African Society of Human Genetics Meeting. Cairo-Egypt. Poster presentation by Farouk.S.
    9. Said M. I., Aziz R. A., Fayed A. A., Mohamed H.S. Ibrahim M. E. (2007).  Novel Polymorphism in a Chemokine Receptor Type 5(CCR5) Under Directional Natural Selection. African Society of Human Genetics Meeting. Cairo-Egypt. Poster presentation by Said M.
    10.  Masri M.A., Elhassan N. M., Mohamed H. S., Wasunna C., Ibrahim ME

Mitochondrial DNA Subtypes in Sudanese Breast Cancer Patients and Healthy Controls. African Society of Human Genetics Meeting. Cairo-Egypt. 2007. Poster presentation by Masri M.A

    1. Elmahdi M.T., Kordofani A.A.,.Mohamed H.S , Ibrahim ME. (2007). Frequency of Glutathione S Transferase T1 and M1 (Gstt1&M1) Null Genotype among Sudanese Leukemia Patients. African Society of Human Genetics Meeting. Cairo-Egypt. Poster presentation by Elmahdi.
    2. Eid N. A., Elzein A. M., Hussein A., Mohammed H. S., Kwiatkowski D., Ibrahim M. E. Candidate Malaria Protective SNPs Shared in Hospital and Population Based Studies. African Society of Human Genetics Meeting. Cairo-Egypt. 2007. Poster presentation by Eid N.

Publications:

1-     Blackwell JM, Goswami T, Evans CA, Sibthorpe D, Papo N, White JK, Searle S, Miller EN, Peacock CS, Mohamed H.S, Ibrahim M.  (2001).  SLC11A1 (formerly NRAMP1) and disease resistance.  Cell Microbiol. 2001 Dec; 3(12): 773-84.

2-     Blackwell, J.M., Searle, S., Mohamed, H.S, White, J.K. (2003). Divalent cation transport and susceptibility to infectious and autoimmune disease: Continuation of the Ity/Lsh/Bcg/Nramp1/Slc11a1 gene story. Immunology Letters. 22;85(2):197-203

3-     Mohamed HS, Ibrahim ME, Miller EN, Peacock CS, Khalil EA, Cordell HJ, Howson JM, El Hassan AM, Bereir RE, Blackwell JM. (2003).  Genetic susceptibility to visceral leishmaniasis in The Sudan: linkage and association with IL4 and IFNGR1. Genes Immun.  4(5):351-5.

4-     Bereir RE, Mohamed HS, Seielstad M, El Hassan AM, Khalil EA, Peacock CS, Blackwell JM, Ibrahim ME.  (2003) Allele frequency and genotype distribution of polymorphisms within disease-related genes is influenced by ethnic population sub-structuring in Sudan. Genetica. 119(1):57-63.

5-     Mohamed HS,  Ibrahim ME, Miller EN, White JK, Cordell HJ, Howson JM,  Peacock CS, Khalil EAG, El Hassan AM, Blackwell JM. (2004).  SLC11A1 (formally NRAMP1) and susceptibility to Visceral Leishmaniasis in The Sudan.  European Journal of Human Genetics.12, 66-74

6-      Blackwell, JM., Mohamed HS., Ibrahim ME. (2004). Genetics and visceral leishmaniasis in the Sudan: seeking a link. Trends in Parasitology. 20(6): 268-74.

7-     Elamin EM, Guerbouj S, Musa AM, Guizani I, Khalil EA, Mukhtar MM, Elkadaro AM, Mohamed HS, Ibrahim ME, Abdel Hamid MM, El Azhari M, El Hassan AM (2005).  Uncommon clinical presentations of cutaneous leishmaniasis in Sudan.  Trans R Soc Trop Med Hyg. 99(11): 803.

8-     Salih MA, Ibrahim ME, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2007). IFNG and IFNGR1 Genes Polymorphisms in Susceptibility to Post kala-azar Dermal Leishmaniasis in Sudan, Genes and Immunity 8, 75-78

9-     Miller NE, Fadal M, Mohamed HS, ElzeinA, Jamieson SE, Cordell HJ., Peacock CS., Fakiola M, Raju M, Khalil EA, Elhassan A, Musa AM, Ibrahim ME and Blackwell JM. (2007). Y Chromosome Lineage- and Village-Specific Genes on Chromosomes 1p22 and 6q27 Control Visceral Leishmaniasis in The Sudan.  PLoS Genetics, 3:5

10- Bayele, H.K., Peyssonnaux, C., Giatromanolaki, A., Arrais-Silva W.A.,  Mohamed, H.S Collins, H., Giorgio, S., Koukourakis, M., Johnson, R S, , Blackwell, J.M., Nizet, V. and Srai, S.K.S.(2007). HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA-forming Microsatellite. Blood. 110, (8): 3039- 3048

11- Farouk S.,Ibrahim ME,Salih MA, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2007).  IL10 Gene Polymorphisms and development of Post-Kala-azar dermal leishmaniasis. In preparation

12-  Mohamed, H., Miller, E.N., White, J.K., Jiang, H-R., Belling, I., Bayele, H.K., Ibrahim, M.E., and Blackwell, J.M. (2007). Sequence analysis of SLC11A1 in relation to its role in infectious and autoimmune diseases. In preparation

Supervision

Main supervisor

1-      Mervat Elshikh “Looking for possible polymorphisms within IL4 promoter region” -Master degree

2-      Badria Mohamed Said “Genotyping of Nitric Oxide Synthase promoter polymorphisms among selected ethnic group in Eastern Sudan” – Master degree

3-      Ahmed Elnazeer ‘Genetics factors determining the severity of P. falciparum infection’- Master degree

4-      Ahmad Adlan Mohamed “Prevalence of Plasmodium falciparum  eba-175 gene in two different populations)  Master Degree

Graduated MSC students:

5-      Mohmed Ahmed Mohmed Salih “Role of IFNgR1 and IFNg polymorphisms in susceptibility to PKDL in selected Sudanese Population” July–2006

6-       Shimaa Farouq A/rahman “IL10 genes polymorphisms and susceptibility to PKDL in selected Sudanese Population” May-2007.

Co-Supervisor

1-      Mather Abd ElWahab “Immunogenetics of cutaneous leishmanisas ” Master degree

2-      Manal Gamal Abdu “ leishmania virulence genes” – PhD degree

3-      Neeven Frank “Immuno respone towards Mycobacterium Tuberculosis” Master degree

Examination of post graduates

    • I have examined three master degree students (University of Khartoum) as internal examiner

Experiences and Skills:

I- Laboratory

DNA extraction from different biological material,. RNA extraction, RT-PCR., ARMS –PCR, RFLP, Southern blotting, Gel and PCR purification. Microsatelite, analysis-using Gene scan -Genotyper softwares. Cloning, generation of construct, muagensis, transfection,  Luciferase reporter gene assay, Real Time PCR, Sequencing. Stimulation assay. Culture of:  Cell line, Peripheral blood Mononuclear Cell (PBMC), Malaria and leishmania parasite. Identification and dissection of sandfly, 

II Genetic analysis:

  • Pedigree inconsistency using Pedcheck .
  • Association analysis using; TDT, TDTphase.
  • Linkage Disequilibrium Hardy-Weinberg deviation analysis using Haploview.
  • Linkage analysis using SPLINK, ALLEGRO

III Bioinformatics

  • Primers designing, Restriction site analysis, sequence analysis using Sequence Navigator and Standen package (pregap, gap4).
  • Sequences alignment (Blast). Alibaba and MatInspector, for identification of putative Transcription site.
  •  Ensemble, NCBI and Genome Browser for gene annotation
  •  Vector NTI

III Computer

  • Windows, UNIX and Linux, Microsoft Office, Word, Excel,. Epinfo, SPSS programs.
  • Internet regular user mainly for information search and email

Reviewer:

·        Molecular Biology Reports- International Journal –2007

Memberships

    • African Society of Human Genetics
    • Sudanese Society of Clinical Biology