Name

Title

Place of Birth

Nationality

Address

Institute of Endemic Diseases. University of Khartoum. P. O. Box 102.

E-mail hibasalah@iend.org:  Fax:  002491-83779712.

Tel.Office: 002491-83793268,Mobile 0912605221

Qualification

Current Post

Assistant Professor, Department of Molecular Biology. Institute of Endemic Diseases. University of Khartoum

Education

• 1975-1987 Tuti Primary, Intermediate and Higher Secondary Schools.  

• 1987- 1993:    BSc.(Hons), Second Class Division One, Zoology, faculty of Science, University of Khartoum

• 1995-1997:   MSc. Zoology, University of Khartoum entitled: A Cross-sectional Study of Cutaneous Leishmaniasis in Tuti Island Supervised by Prof Ahmed M. El Hassan and Dr Sadia A Youins.

• 1998-2002:   Ph.D,  Molecular Biology.  Institute of Endemic Diseases University of Khartoum entitled: The role of Host Genetics in Susceptibility to Kala-azar in The Sudan. Supervised by:  Prof. J.Blackwell, University of Cambridge and Dr Muntaser E. Ibrahim.  Institute of Endemic Diseases, University of Khartoum.

Last Post Held

• 1993-1996:   Teaching assistant on part time bases (Faculty of Science, Faculty of   Education, University of Khartoum- University of Juba- University of Bahr El Gazal)
• 1994-1997:   Teaching assistant University of El Neelain, Sudan
• 1998-2000:   Lecturer, University of El Neelain, Sudan
• 2000- 2002:  Lecturer.  Institute of Endemic Diseases, University of Khartoum
• 2002-2003:Research assistant during my Ph.D in the Welcome Trust Collaborative Grant (Role of Host genetics in Susceptibility to Kala-azar In Sudan) between university of Khartoum (Dr. Muntaser E. Ibrahim ) and University of Cambridge ( Prof. Blackwell).
• March-October 2002:  Post doctoral fellowship. Cambridge Institute for Medical Research. University of Cambridge.
• June 2002- till now Assistant Professor-Department of Molecular Biology –Institute of endemic Diseases, University of Khartoum.

Ongoing and Conducted Researches

• Frequency of sugar feeding of Phlebotomus Papatasi in Surogia area. (1993). Graduation dissertation. Supervisor Dr. Dia Eldin Elnaiem.  Faculty of Science University of Khartoum   

• Epidemiological study of cutaneous leishmaniasis in Tuti Island-Khartoum looking for the prevalence of the disease in human and sandflies (MSc-Research)-1996-1998)
• Genetic susceptibility to visceral leishmaniasis –Candidate gene approach (1999-2002). PhD research
• Diagnosis of Leishmaniasis using PCR  (1996-1998)
• Functional and Genetic analysis of NRAMP1 gene using Real time PCR, reporter assay and sequencing (2003-2007).
• Looking for possible reservoir host for visceral leishmaniasis by investigating natural infection and experimental infection in dogs (2006-ongoing)
• Immunogenetics of cutaneous leishmanisas in newly inhabited area in Khartoum (2007-ongoing)
• Screening of known cytokine, nitric oxide and complement receptor one gene polymorphisms in Sudanese from malaria endemic areas
• Possible genes control susceptibility to Mycetoma, using SNP chips (On going research)

Main Research Interest

• Host –Parasite interaction

• Genetics susceptibility to infectious Diseases in particular to Visceral Leishmaniasis (VL), Post-kala-azar Dermal Leishmaniasis (PKDL) and Malaria
• Looking for possible functional role of different gene polymorphisms and its role in differential gene expression using Reporter assay, Real time PCR.

Courses and Training

• Molecular Biology techniques in Research and Diagnosis of Infectious Diseases (1996). Institute of Endemic Diseases, University of Khartoum
• Biostatistics, using Statistical Package for Social Studies (SPSS) (1997). Tropical Medicine Institute. Sudan 
• Introduction to Immunology and Molecular Biology (1997).  Institute of Endemic Diseases, University of Khartoum
• Genotyping and Linkage Analysis Course (1999).  Human Genome Resource Centre. Hinxton. Cambridge. UK
• Introduction to Bioinformatics (2000). Cambridge University. UK.
• Training at Cambridge Institute for Medical Research (Part of my Ph.D). (1999-2002). University of Cambridge. Funded by WHO/TDR and Wellcome Trust.
• WHO/TDR advance course in Immunology and biotechnology for infectious diseases, (2003.). Lausanne- Switzerland, Annecy- France.
• Training from 2004-2007, three months every year,  at Cambridge Institute for Medical Research- University of Cambridge. UK.

• Training course for one month at Institute for Staff Skills Development -University of Khartoum (2006).
• Functional Genomic of Malaria Parasites (Biotec, Thiland ). March -2007.  Funded by WHO/TDR and BioMalPa
• Best Practices in Ethical Review. 24-26 July 2007. Organized by HAT Platform, Good Clinical Practice Alliance – Europe and Tropical Medicine Institute, NCR, Khartoum-Sudan -Khartoum -Sudan

Oral and Poster Presentation in Meetings

• Molecular Epidemiological Study of Cutaneous Leishmaniasis in Tuti Island. (1998). Oral Presentation.  Institute of Endemic Diseases – Khartoum -Sudan.
• Role of Candidate Genes in Susceptibility to Kala-azar in the Sudan. 2001. Poster.  Keystone Symposium USA
• Role of NRAMP1 in Susceptibility to Kala-azar. Oral presentation in the 6th International Meeting on Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, Pasteur Institute, Paris, France, 23-27 July 2002.
• Role of Host Genetics in Susceptibility to Kala-azar. (2002). Oral presentation. In Genetics of the population of the Sudan and East Africa Meeting. Institute of Endemic Diseases. University of Khartoum. Sudan.
• Salih MA, Ibrahim ME, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2006). IFNG and IFNGR1 Genes Polymorphisms in Susceptibility to Post kala-azar Dermal Leishmaniasis in Sudan. Poster presentation by Salih MA in African Society of Human Genetics meeting –Addis Ababa, Ethiopia-
• Badria MS, ElHassan IM., Ibrahim ME and Mohamed HS.,. Genotyping of nitric oxide synthase 2 polymorphisms  in different ethnic groups in Eastern Sudan.  Poster presentation in 4th MIM Pan-African Malaria conference-2005 in Cameroon by Badria MS.

Badria MS, ElHassan IM., Ibrahim ME and Mohamed HS.,. Prevalence of nitric oxide gene polymorphisms in two populations.  XI international Congress of Parasitology ,  Glasgow –2006.  Poster presentation by Badria MS

Grants Awarded

• WHO/ TDR training fellowship for 8 months (1999) in Cambridge University.

• Wellcome Trust Research Development Award (2004-2007). Genetics and Functional analysis of SLC11A1 (formerly NRAMP1) polymorphisms in susceptibility to visceral leishmaniasis in the Sudan.  In collaboration with Prof. Blackwell University of Cambridge. UK, (₤90,000) The Award was included (fund for: equipment to the Institute, consumables, visits to Cambridge university 3 months every year for research).

• University of Khartoum Research fund for one years (2007) (Genetics and immunological factors determining the severity of P. falciparum infection. (5000 SDG).

• WHO capacity strengthening program grant for faculty of Science “ investigation of possible reservoir host in Eastern Sudan “ Co-investigator.

• Royal Society Pfizer Award (2007). Recognition of my work on the genetic basis of susceptibility to visceral leishmaniasis in the Sudan. The prize support young African scientist in biomedical field. ₤60,000 fund for the research entitled “Functional Genomic of Visceral leishmaniasis and PKDL in the Sudan”.

Teaching Experiences

• General Zoology- Practical–first Year (1995-2000) - AL-Neelain University.

• General Zoology- Practical–first Year (1994-2000)-Faculty of Science- U of K, Faculty of   Education- U of K, University of Juba- University of Bahr El Gazal (on part-time bases).

• Genetics-Practical – 2^nd year - AL-Neelain University.
• Animal physiology -Practical - 4^th Year –AL-Neelain University.

·  Vertebrate course-Theory-Practical– 2^nd year- (1999)- AL-Neelain University.

·  Host-parasite interaction- Theory -Third year (1999)-AL-Neelain University.

· Molecular Biology course - Qualifying year - Institute of Endemic Diseases-University of Khartoum –2002 –to date

· Molecular biology course -4^th year - Al-Neelain University- 2004

·   Statistical Genetics Theory and Practical Institute of Endemic Diseases-2005- to date

· Cell Molecular Biology course  – Master of Molecular Medicine –Institute of Endemic Diseases- (2007)

· Genetic susceptibility to infectious diseases- Master degree of molecular medicine –Institute of Endemic Diseases- (2007)

·Statistical Genetics- Master of molecular medicine –Institute of Endemic Diseases (2007).

·Bioinformatics and statistical genetics. University of Elneelain February –2007

·Introduction to Bioinformatics course –2006-‏2007‏‏‏

· Molecular biology -First year- National college for medical and technical studies-2007.

Administrative Responsibilities

• Examination 

• Postgraduate Students Coordinator (2006-present)

Organization of Workshop and Courses

Capacity Building in the Institute From the Wellcome Trust

RNA laboratory:

Cell culture facilities:

  –20 oC freezer, laminar flow hood, CO2 incubator, distiller, reagents and consumable

Experiences and Skills:

I- Laboratory

DNA extraction from different biological material,. RNA extraction, RT-PCR., ARMS –PCR, RFLP, Southern blotting, Gel and PCR purification. Microsatelite, analysis-using Gene scan -Genotyper softwares. Cloning, generation of construct, muagensis, transfection,  Luciferase reporter gene assay, Real Time PCR, Sequencing. Cell line and Peripheral blood Mononuclear Cell (PBMC) and Malaria parasite and leismania culturing. Stimulation assay. Identification and dissection of sandfly, 

II Genetic analysis:

• Pedigree inconsistency using Pedcheck .
• Association analysis using; TDT, TDTphase.
• Linkage Disequilibrium Hardy-Weinberg deviation analysis using Haploview.

Linkage analysis using SPLINK, ALLEGR

III Bioinformatics

• Primers designing, Restriction site analysis, sequence analysis using Sequence Navigator and Standen package (pregap, gap4).
• Sequences alignment (Blast). Alibaba and MatInspector, for identification of putative Transcription site.
•  Ensemble, NCBI and Genome Browser for gene annotation
•  Vector NTI

III Computer

• Windows, UNIX and Linux, Microsoft Office, Word, Excel,. Epinfo, SPSS programs.
• Internet regular user mainly for information search and email

Recent Publications:

1-     Blackwell JM, Goswami T, Evans CA, Sibthorpe D, Papo N, White JK, Searle S, Miller EN, Peacock CS, Mohamed H.S, Ibrahim M.  (2001).  SLC11A1 (formerly NRAMP1) and disease resistance.  Cell Microbiol. 2001 Dec; 3(12): 773-84.

2-     Blackwell, J.M., Searle, S., Mohamed, H.S, White, J.K. (2003). Divalent cation transport and susceptibility to infectious and autoimmune disease: Continuation of the Ity/Lsh/Bcg/Nramp1/Slc11a1 gene story. Immunology Letters. 22;85(2):197-203

3-     Mohamed HS, Ibrahim ME, Miller EN, Peacock CS, Khalil EA, Cordell HJ, Howson JM, El Hassan AM, Bereir RE, Blackwell JM. (2003).  Genetic susceptibility to visceral leishmaniasis in The Sudan: linkage and association with IL4 and IFNGR1. Genes Immun.  4(5):351-5.

4-     Bereir RE, Mohamed HS, Seielstad M, El Hassan AM, Khalil EA, Peacock CS, Blackwell JM, Ibrahim ME.  (2003) Allele frequency and genotype distribution of polymorphisms within disease-related genes is influenced by ethnic population sub-structuring in Sudan. Genetica. 119(1):57-63.

5-     Mohamed HS,  Ibrahim ME, Miller EN, White JK, Cordell HJ, Howson JM,  Peacock CS, Khalil EAG, El Hassan AM, Blackwell JM. (2004).  SLC11A1 (formally NRAMP1) and susceptibility to Visceral Leishmaniasis in The Sudan.  European Journal of Human Genetics.12, 66-74

6-      Blackwell, JM., Mohamed HS., Ibrahim ME. (2004). Genetics and visceral leishmaniasis in the Sudan: seeking a link. Trends in Parasitology. 20(6): 268-74.

7-     Elamin EM, Guerbouj S, Musa AM, Guizani I, Khalil EA, Mukhtar MM, Elkadaro AM, Mohamed HS, Ibrahim ME, Abdel Hamid MM, El Azhari M, El Hassan AM (2005).  Uncommon clinical presentations of cutaneous leishmaniasis in Sudan.  Trans R Soc Trop Med Hyg. 99(11): 803.

8-     Salih MA, Ibrahim ME, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2007). IFNG and IFNGR1 Genes Polymorphisms in Susceptibility to Post kala-azar Dermal Leishmaniasis in Sudan, Genes and Immunity 8, 75-78

9-     Miller NE, Fadal M, Mohamed HS, ElzeinA, Jamieson SE, Cordell HJ., Peacock CS., Fakiola M, Raju M, Khalil EA, Elhassan A, Musa AM, Ibrahim ME and Blackwell JM. (2007). Y Chromosome Lineage- and Village-Specific Genes on Chromosomes 1p22 and 6q27 Control Visceral Leishmaniasis in The Sudan.  PLoS Genetics, 3:5

10- Bayele, H.K., Peyssonnaux, C., Giatromanolaki, A., Arrais-Silva, W.A., Collins, H., Giorgio, S., Koukourakis, M., Nizet, V., Mohamed, H.S., Blackwell, J.M., Johnson, R S. and Srai, S.K.S.(2007). HIF-1a regulates heritable variation in SLC11A1 expression phenotypes relevant to innate immunity and inflammation. Accepted subject to revisions in Blood.

11- Farouk S.,Ibrahim ME,Salih MA, Blackwell JM, Miller Nancy, Khalil EAG, ElHassan AM, Musa AM and Mohamed HS. (2007).  IL10 Gene Polymorphisms and development of Post-Kala-azar dermal leishmaniasis. In preparation

12-  Mohamed, H., Miller, E.N., White, J.K., Jiang, H-R., Belling, I., Bayele, H.K., Ibrahim, M.E., and Blackwell, J.M. (2007). Sequence analysis of SLC11A1 in relation to its role in infectious and autoimmune diseases. In preparation

Students under supervision

Main supervisor

1-     Mervat Elshikh “Looking for possible polymorphisms within IL4 promoter region” -Master degree

2-     Badria Mohamed Said “Genotyping of Nitric Oxide Synthase promoter polymorphisms among selected ethnic group in Eastern Sudan” – Master degree

3-     Ahmed Elnazeer ‘Genetics factors determining the severity of P. falciparum infection’- Master degree

4-     Ahmad Adlan Mohamed “Prevalence of Plasmodium falciparum  eba-175 gene in two different popultions)  Master Degree

Co-Supervisor

1-     Mather Abd ElWahab “Immunogenetics of cutaneous leishmanisas ” Master degree

2-     Manal Gamal Abdu “ TB proteomic” – PhD degree

Graduated MSC students:

1. Mohmed Ahmed Mohmed Salih “Role of IFNgR1 and IFNg polymorphisms in susceptibility to PKDL in selected Sudanese Population” July–2006

2.  Shimaa Farouq A/rahman “ IL10 genes polymorphisms and susceptibility to PKDL in selected Sudanese Population” May-2007.

Internal Examiner

Molecular Biology Reports- International Journal -2007

• African Society of Human Genetics
• Sudanese Society of Clinical Biology